Gaining a thorough understanding of the mechanisms behind fat cell production is, thus, essential for the development of novel treatment strategies. While past studies focused on individual molecular layers, we recognize the importance of considering higher network connectivity levels, particularly lipid feedback controlling the master regulator of adipogenesis, PPARG. Our approach integrates advanced lipidomics and proteomics techniques, monitoring PPARG and the lipidome during perturbations in vitro. Through innovative multiomics strategies and high-throughput imaging, we dissect feedback networks and identify lipid regulators of PPARG. This research not only advances scientific knowledge but also holds promise for future obesity drug development, potentially revolutionizing treatment approaches.